醋酸汞对大鼠支持细胞某些功能的影响

Theefectsofmercuryacetateoncertainfunctionsofratsertolicelinprimaryculture

研究结果发现，Ｈｇ２＋（１０－８～１０－５ｍｏｌ／Ｌ）使得处理２４小时的Ｓｅｒｔｏｌｉ细胞乳酸含量显著升高，并具有明显的剂量反应关系；Ｈｇ２＋（１０－６～１０－５ｍｏｌ／Ｌ）使得丙酮酸含量也明显升高。Ｈｇ２＋（１０－６ｍｏｌ／Ｌ）使得处理６～２４小时的Ｓｅｒｔｏｌｉ细胞乳酸含量均显著高于对照组水平，具有明显的时间反应关系；但丙酮酸含量仅在２４小时才显著升高。Ｈｇ２＋（１０－８～１０－５ｍｏｌ／Ｌ）使得处理２４小时的Ｓｅｒｔｏｌｉ细胞内ｃＡＭＰ含量显著下降，具有明显的剂量反应关系。而醋酸锌（Ｚｎ２＋）对培养的Ｓｅｒｔｏｌｉ细胞的上述观察指标均无影响。

nthisstudy,theefectofMercuryAcetate(Hg2+)ontheconcentrationsofprimaryculturedSertolicel′slactate,pyruvateandintracelularcAMPwereexamined.TheresultsdemonstratedthatHg2+(10-8～10-5mol/L)resultedinadose-relatedin-creaseconcentrationoflactateovera24hrtreatmentperiod.Hg2+(10-6～10-5mol/L)alsocausedadose-relatedincreaseconcentrationofpyruvateovera24hrtreatmentperiod.Hg2+(10-6mol/L)resultedinatime-relatedincreaseconcentrationoflactateover6,12,18,and24hrtreatmentperiods,andonlyaincreaseconcentrationofpyruvateover24hrtreatmentperiods.Hg2+(10-8～10-5mol/L)resultedinadose-relateddecreaseintracelularcAMPconcentrationovera24hrtreatmentperiod.OurresearchshowedthatZincAcetate(Zn2+),whichisknowntobeessentialforthemaintenanceofgerminalepitheliuminthetestes,didnotcauseanyvarietiesoftheindicesshowedabove.Sowecanusetheseindicesasbiochemi-calmarkerstoevaluatethetoxicefectsofHg2+onSertolicelofthetestes.