人组织型纤溶酶原激活剂(t-PA)在转基因烟草中的表达

Expression of Human Tissue-Type Plasminogen Activator in Transgenic Tobacco

实验室构建了携带有人组织型纤溶酶原激活剂(tissue plasminogen activator,t-PA)植物表达载体并转化入根瘤农杆菌GV3101。该农杆菌以叶盘法转化烟草,建立表达t-PA的转基因烟草体系。通过对转化植株进行Southern blotting,RT-PCR,Western blotting和纤维蛋白琼脂糖平板(fibrin-agarose plate assay,FAPA)法检测,结果显示,在转基因烟草中成功地表达出有活性的人t-PA。

The plant expressing vectors containing human t-PA gene was constructed and transformed in into agnobacterium GV3101. The transgenic tobacco system expressing human tissue plasminogen activator （t-PA） was established by infecting tobacco with the agnobacteriurn by leaf disk method. The transgenic efficiency was evaluated by Southern-blotting, RT-PCR, Fibfin-agarose plate assay （FAPA） and Western-blotting. Results suggested that bio-active human t-PA was successfully expressed in the transgenic tobacco system.