以海藻酸钙为载体固定化谷氨酰胺转胺酶及其性质的研究

Preparation and its properties of the immobilized transgluminase with calcium alginate as the support

以海藻酸钙为载体、戊二醛为交联剂,对谷氨酰胺转胺酶进行固定化。研究了海藻酸钠浓度、戊二醛浓度、酶浓度、缓冲液pH值及交联温度对固定化酶的影响,并对固定化酶酶促反应的最适温度、pH值等进行了测定。结果表明:海藻酸钠浓度为5.0%、戊二醛浓度为1.0%、酶浓度为20%、缓冲液pH值为7.0、在35℃条件下固定化效果最好。固定化酶的pH值稳定性、热稳定性都比游离酶显著提高。

The transgluminase was immobilized on calcium alginate carried by cross-link with glutaraldehyde. The effects of sodium alginate concentration,amount of glutaraldehyde concentration,amount of transgluminase concentration,amount of pH value the enzyme and temperature on immobilization were analyzed, The reaction conditions（optimum pH and temperature） of the immobilized transgluminase were studied. The results showed that the under the conditions of 5.0% sodium alginate, 1.0% glutaraldehyde, 20% transgluminase and 35~6 ,pH value7,0.The stabilities of the pH value and thermostability of the immobilized enzyme are obviously higher than those of native transgluminase.