摘要
采用体外全胚胎培养模型和显微镜检查方法,对接触不同剂量氯化甲基汞的离体SD大鼠胚胎进行了病理学观察。结果表明:甲基汞浓度为0.1μg/ml时,神经管上皮层细胞脱落,室管膜层变薄和排列无序;尿生殖窦、肝脏等亦出现一定病理变化。随着染毒剂量的增加,上述组织的病理改变更为显著,虽明显的剂量效应关系。0.4~0.8μg/ml甲基汞还可诱发脑室、心脏、前肢芽等组织器官发生病理学改变。甲基汞浓度≤0.8μg/ml时未见对卵黄囊结构的明显损害,揭示甲基汞能迅速通过卵黄囊胎盘而直接攻击胚胎,这就从病理学的角度证明。
Morphological observations of teratogenicity of methylmercury chloride(MMC) on 9 5 day isolated Sprague Dawley rat embryos were studied with whole embryo culture in vitro and optical microscopy.The results showed:when dose of MMC was 0 1μg/ml,not only epithelial cells of neural tube lost and ependymocytes were out of order but urinogenital sinus and liver appeared some pathological changes.These changes became more apparent and showed dose effect relationship as MMC levels rose.At 0 4-0 8μg/ml MMC groups the pathological damages of brain,heart and fore limb bud could be observed under optical micrescopy.Obvious morpholog
出处
《现代预防医学》
CAS
1996年第4期193-194,共2页
Modern Preventive Medicine
基金
国家自然科学基金