摘要
目的:研究明胶海绵作为颞下颌关节组织工程支架材料应用的可行性。方法:采用胶原酶阶段消化法获取兔髁突软骨中的纤维软骨细胞,取经碱性成纤维细胞生长因子(bFGF)扩增的第3代细胞与已用多聚赖氨酸修饰的明胶海绵复合培养,进行噻唑蓝(MTT)比色及光镜、扫描电镜观察。结果:纤维软骨细胞于明胶海绵孔壁内牢固粘附,5d开始增殖,4W时有大量细胞生长于明胶海绵上。结论:明胶海绵表现出良好的亲水性、细胞吸附性和体外生物相容性,可作为组织工程细胞培养支架材料用。
Objective： To investigate the possibility of amnion as cell scaffold in tissue engineering of temporomandibular joint. Methods： Rabbit mandibular condylar fibrocartilage was digested＇by collagenase,and cells of the second generation proliferated by TGF-β1 were cultured onto amnion. Observation was made with MTI＇, light microscope lage cells attached to amnion closely and , and electronic scanning microscopy. Results： Fibrocartibegan to proliferate on the 3rd day. In 3 weeks after the transplantation, a lot of cells grew well on amnion. Conclusion： Amnion has good water adsorptivity and biocompatibility with fibrocartilage cells. It can be used as scaffold for tissue engineering of temporomandbular joint.
出处
《贵阳医学院学报》
CAS
2006年第5期393-395,共3页
Journal of Guiyang Medical College
基金
贵州省优秀科技人才省长基金资助项目(S2005-2)
关键词
颞下颌关节
下颌骨髁状突
软骨
细胞培养
明胶海绵
吸收性的
组织工程
temporomandibular joint
mandibular condyle
cartilage
cell culture
gelatin sponge, absorbable
tissue engineering
