蛋白激酶CK2α在人喉癌细胞Hep-2中的表达及意义

THE EXPRESSION OF PROTEIN KINASE CK2α IN HUMAN LARYNGEAL CARCINOMA CELL LINE HEP-2 AND ITS SIGNIFICANCE

目的研究蛋白激酶CK2α(Protein kinase CK2α,PK-CK2α)在人喉癌细胞Hep-2中的表达,探讨其与喉癌之间的关系。方法体外培养Hep-2细胞和Hacat细胞,应用半定量逆转录-聚合酶链反应(RT-PCR)技术分别检测蛋白激酶CK2αmRNA在Hep-2细胞和Hacat细胞中的表达;应用免疫细胞化学技术分别检测蛋白激酶CK2α蛋白在Hep-2细胞和Hacat细胞中的表达。结果蛋白激酶CK2αmRNA在Hep-2细胞中高表达,在Hacat细胞中低表达,差异有统计学意义(P<0.01)。蛋白激酶CK2α蛋白在Hep-2细胞中高表达,在Hacat细胞中低表达,差异有统计学意义(P<0.01)。结论蛋白激酶CK2α过表达可能与喉癌的发生和发展有关。

Objective To study the expression of protein kinase CK2α in human laryngeal carcinoma cell line Hep-2 cells and its significance. Methods The expression level of protein kinase CK2α mRNA in Hep-2 cells and Hacat cells was measured with semi-quantitative reverse transcription polymerase chain reaction （RT-PCR）. The expression of protein kinase CK2α protein in Hep-2 cells and Hacat cells was measured with immunocytochemical techniques. Results The expression of both protein kinase CK2α mRNA and protein were significantly stronger in Hep-2 cells than in Hacat cells （P〈0. 01）. Conclusion Formation and development of laryngeal carcinoma may be related with the overexpression of protein kinase CK2α.