Cyclin G1、MDM2和p53在胃腺癌组织中的表达及相关性研究

EXPRESSIONS AND CORRELATIONS OF CYCLIN G1,MDM2 AND p53 PROTEIN IN GASTRIC CANCER

目的探讨细胞周期蛋白G1(cyclinG1)、鼠双微体基因(MDM2)和p53在胃腺癌组织中的表达意义及相关性。方法采用免疫组织化学方法(SP法)检测54例胃腺癌组织中cyclinG1、MDM2和p53的表达,以20例正常胃粘膜组织作为对照。结果cyclinG1、MDM2、p53在胃腺癌组织中的阳性表达率分别为62.96%、53.70%、44.44%,而在正常胃粘膜组织中的阳性表达率分别为0%,15.00%,0%,两者比较差异均有显著性(P均<0.05),cyclinG1、MDM2在胃腺癌中的表达与肿瘤的分化程度相关(P均<0.05)。胃癌组织中MDM2蛋白的表达与p53的表达呈正相关(r=0.307),而cyclinG1蛋白的表达与p53的表达无明显相关性。结论CyclinG1、MDM2、p53的阳性表达在胃癌的发生、发展过程中起着重要的的作用。MDM2可能是通过调控p53的活性而促进胃癌的发生、发展,cyclinG1可能以不依赖p53的途径发挥作用。

Objective To study the expressions and correlations of cyclin G1, MDM2 and p53 protein in gastric carcinoma. Methods The expressions of cyclin G1. MDM2 and p53 protein in 54 cases of gastric carcinoma were detected by immunohistochemical methods. Results The positive rates of cyclin G1. MDM2 and p53 protein expressions in gastric carcinoma were 62. 96%. 53. 70% and 44. 44% respectively, while the expressions of cyclin G1 and p53 in normal tissue were negative, and the positive rate of MDM2 in normal tissue was 15%. There were significant differences between them （P〈0. 05）. The positive exepression rate and intensity of cyclin G1 and MDM2 in gastric cancinoma was significantly correlated with tumor differentiation. Conclusion Cyclin G1, MDM2 and p53 might play an important role in the occurrence and development of gastric carcinoma.