银杏叶提取物对巨噬细胞呼吸爆发、炎性细胞因子和COX-2 mRNAs及蛋白表达的影响

EFFECTS OF EGB761 ON THE RESPIRATORY BURST AND THE EXPRESSIONS OF INFLAMMATORY CYTOKINES AND COX-2 mRNA/PROTEIN IN MACROPHAGES

目的探讨银杏叶提取物(EGb761)对小鼠巨噬细胞呼吸爆发及IL-1β、IL-6、TNF-α、COX-2mRNAs和蛋白表达的影响。方法以佛波酯刺激巨噬细胞产生呼吸爆发,用化学发光分析和电子顺磁共振检测呼吸爆发产生的活性氧;以脂多糖(LPS)诱导巨噬细胞IL-1β、IL-6、TNF-α、COX-2表达,用RT-PCR检测IL-1β、IL-6、TNF-α、COX-2mRNAs表达,ELISA法检测IL-1β、IL-6、TNF-α、COX-2蛋白表达。结果银杏叶提取物能清除巨噬细胞呼吸爆发产生的超氧阴离子自由基、下调LPS诱导巨噬细胞IL-1β、IL-6、TNF-α、COX-2 mRNAs和蛋白表达。结论银杏叶提取物对巨噬细胞产生呼吸爆发和LPS诱导巨噬细胞IL-1β、IL-6、TNF-α、COX-2 mRNAs和蛋白表达有明显的抑制作用。

Objective To study the effects of Ginkgo biloba extract （EGb761） on the respiratory burst and the expressions of IL-1β, IL-6, TNF-α and COX-2 mRNAs and proteins in macrophages. Methods The respiratory burst was produced in macrophages by using phorbol, and the effects of EGb761 on the generation of superoxide anion in stimulated primary macrophages were studied by chemiluminenscence and electron paramagnetic resonance （EPR） techniques. The expression of IL-1β, IL-6, TNF-α and COX- 2 were induced in macrophages by using LPS, and the effects of EGb761 on the expression of IL-1β, IL-6, TNF-α and COX-2 mRNAs and proteins in stimulated primary macrophages were analysed by reverse tran- scription-polymerase chain reaction （RT-PCR） and enzyme-linked immunosorbent assay （ELISA）. Results EGb761 scavenged the endogenous superoxide anion dose-dependently, and inhibited the expression of IL-1β, IL-6, TNF-α and COX-2 mRNAs and proteins in stimulated primary macrophages. Conclusion EGb761 can significantly inhibit the expression of IL-1β, IL-6, TNF-α and COX-2 mRNAs and proteins in LPS-induced macrophages.