摘要
目的检测caspase-3在老年豚鼠耳蜗的表达。方法实验分两组:实验组和对照组,实验组豚鼠年龄为33至35个月之间,对照组豚鼠年龄为2至3个月。用免疫组织化学方法检测caspase-3在两组豚鼠耳蜗的表达。结果Caspase-3在实验组耳蜗的表达呈阳性,阳性区域主要存在于耳蜗螺旋神经节细胞。在对照组耳蜗的表达呈阴性。结论Caspase-3在老年豚鼠耳蜗螺旋神经节细胞中呈阳性表达,提示caspase-3在豚鼠耳蜗老化过程中起重要作用。
Objective To detect the expression of caspase-3 in the cochlea of old aged guinea pigs. Methods Guinea pigs of the experimental group were 33 to 35 months old, and those of the control group were 2 to 3 months old. Caspase-3 was detected in the cochlea of both groups with the method of immunohistochemistry. Results The expression of caspase-3 was positive in the cochlea of experimental guinea pigs, and the caspase-3 positive area was in spiral ganglion cells, while caspase-3 was negative in the control group. Conclusion The expression of caspase-3 was positive in the spiral ganglion cells of aged guinea pig, which suggests that caspase-3 plays a very important part in the process of aging of the guinea pig＇s cochlea.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2006年第5期547-549,共3页
Chinese Journal of Histochemistry and Cytochemistry
