玻璃化法保存微囊化大鼠原代肝细胞的研究

Study of vitrificantion in preseration of microencapsuled rats hepatocytes

目的探讨采用玻璃化法低温保存微囊化肝细胞的效果,优化最佳冷冻条件。方法使用3 3%,3 7%,4 0%3种不同浓度的乙二醇保护剂,以不同的冷冻速度低温保存微囊化的肝细胞,并检测冷冻后微囊内的肝细胞功能。结果4 0%乙二醇浓度的保护剂配方能最有效保护肝细胞,3种冷冻速度的冷冻效果相同,冷冻后肝细胞P 4 5 0功能和尿素合成功能与冷冻前相同。结论玻璃化法能够有效的保存微囊化的肝细胞。

Objective To study the effect of cryopresevation of vitrified microencapsul in preserving the rat hepatocytes, and to select the best cryopreservation condition of hepatocytes. Methods 33 % , 37 % , 40 % ethyl hydroxide was respectively used as the cryopreserving agent of hepatocytes, and the effect of different speeds of cryopreservation on hepatocytes conservation was tested. Results The best cryopreseration agent of hepatocytes was 40% ethyl hydroxide ; the effect of different speeds of cryopreservation on hepatocytes preservation was the same. The P450 activation and urea synthesis function of cryopreserved hepatocytes were as same as before and after cryopresevation. Conclusions Vitrification can presere the microencapsuled rats hepatocytes effectively.