明胶酶、CD44v6在结直肠癌中的表达及意义

Expression of gelatinase and CD44v6 in colorectal carcinoma

目的探讨明胶酶在结直肠癌中的表达及其CD44v6在结直肠癌侵袭转移过程中的关系。方法采用明胶酶谱(gelzymography)和S-P免疫组化对50例结直肠癌癌组织和相应正常组织中的明胶酶、CD44v6进行检测。结果明胶酶在结直肠癌中的表达水平显著高于正常组织(P<0·05);明胶酶与结直肠癌的Duke’s分期呈同步的正相关(P<0·05);明胶酶在CD44v6强阳性组中表达量升高显著(P<0·05)。结论明胶酶与结直肠癌的侵袭转移性有着密切的关系,不仅在癌细胞的酶解,而且在癌细胞的黏附、运动过程中有可能发挥着重要作用。

Objective To explore the expressions of gelatinase and CD44v6,and the relation between them in the process of invasion and metastasis in colorectal carcinoma. Methods Gel zymography and S-P immunohistochemistry were used to examine the expression of gelatinase and CD44v6 in 50 cases of colorectal carcinoma and the corresponding normal tissues （ 〉 5cm far from the tumoral loci） .Results The contents of gelatinase in colorectal carcinoma were higher than those in normal tissue（ P 〈0.05）. The expression of gelatinase was highly related to Duke＇s stage of colorectal carcinoma（ P 〈 0.05）. With the high expression of gelatinase, the expression of CD44v6 in strongly positive group was higher than that in the negative and weakly positive groups（ P 〈 0.05）. Conclusion There existed close relation between gelatinase and biological action of invasion and metastasis of colorectal carcinoma. Gelatinase not only play the important role in the process of zymolysis, but exerts some function in the process of adhesion and translation of colorectal carcinoma cell.