端粒酶催化亚单位和c-myc在喉鳞状细胞癌组织中的表达及意义

Expression of telomerase reverse transcriptase and its relationship with expression of c-myc in laryngeal squamous cell carcinomas

目的:研究喉鳞状细胞癌组织中端粒酶催化亚单位(hTERT)和c-myc的表达及其与喉鳞状细胞癌临床病理参数的关系。方法:应用免疫组织化学法检测39例喉鳞状细胞癌组织和10例声带息肉组织中hTERT和c-myc蛋白的表达,并分析喉鳞状细胞癌组织中hTERT和c-myc蛋白的表达与肿瘤各项临床病理参数的关系。结果:39例喉鳞状细胞癌组织中hTERT、c-myc蛋白阳性表达率分别为84.6%(33/39)和82.1%(32/39);10例声带息肉组织中hTERT蛋白均未见阳性表达,c-myc蛋白阳性表达率为10.0%(1/10),经统计学分析hTERT和c-myc蛋白在喉鳞状细胞癌组织与声带息肉组织中的表达差异有统计学意义(均P<0.01)。喉鳞状细胞癌组织中hTERT蛋白阳性表达与癌组织的临床分期、肿瘤分化程度及颈淋巴结转移之间均无相关性。喉鳞状细胞癌组织中hTERT和c-myc蛋白阳性表达之间存在显著正相关关系。结论:hTERT的激活及c-myc的过度表达在喉鳞状细胞癌的发生中起重要作用,c-myc基因可能是hTERT激活的重要调控因子。

Objective：To investigate the expression of human telomerase reverse transcriptase （hTERT） and c-myc and their relationship with clinicopathological parameter in laryngeal squamous cell carcinoma（LSCC）. Method： The expression of hTERT protein and c myc protein in 39 LSCC specimens and 10 vocal cord polyp specimens were detected by immunohistochemistry. The correlation between the expression of hTERT protein and c-myc protein and their relationship with clinicopathological parameter were analyzed. Result：The positive rate of hTERT protein and c myc protein expression in 39 LSCC was 84.6%（33/39） and 82.1%（32/39）, respectively. The positive rate of hTERT protein and c myc protein expression in 10 vocal cord polyps was 0（0/10） and 10.0% （1/10） respectively. Statistical tests showed that hTERT and c myc protein expression was significantly higher in LSCC than that in vocal cord polyps. Spearman rank correlation analysis revealed that the expression of c-myc protein was great significantly related to that of hTERT protein （correlation coefficient r = 0.541, P 〈0.01 ）. No statistically association was observed among the frequency of hTERT expression and TNM stages, degree of differentiation or lymph nodes metastases. Conclusion： The activation of hTERT may be a key step in the pathogenesis of LSCC and the expression of hTERT was associated significantly with that of c-myc gcne. C-myc is probably an important control factor in the activation and regulation of hTERT expression.