DF3调控下的白喉毒素A片段对人乳腺癌细胞的特异性杀伤作用

Specific Lethal Effect Of Diphtheria Toxin A Fragment Under Control Of DF3 Promotor for Human Breast Cancer Cells

目的:研究新构建的含人乳腺癌DF3启动子和白喉毒素A片段的重组表达载体PGL3-DF3-DTA对人乳腺癌细胞的影响。方法:构建重组表达载体PGL3-DF3-DTA,并用其转染DF3阴性和阳性的乳腺癌细胞。采用RT-PCR法检测DTA在乳腺癌细胞中的表达,通过MTT法测定PGL3-DF3-DTA在体外对乳腺癌细胞生长的影响。建立裸鼠动物模型观察PGL3-DF3-DTA在体内对乳腺癌细胞的杀伤效应。结果:重组表达载体PGL3-DF3-DTA在DF3阳性的乳腺癌细胞中高表达,并在体内外均能发挥特异性杀伤作用。结论:重组表达载体PGL3-DF3-DTA能对DF3阳性的乳腺癌细胞产生特异性杀伤作用。

Objective： To study the effects of recombinant expression vector containing human breast cancer DF3 promotor and diphtheria toxin A fragment on human breast cancer cells. Methods： Constructing recombinant expression vector PGL3-DF3-DTA and transfecting it into human breast cancer cells of DF3 positive and negative. By means of RT-PCR to measure the expression of DTA in human breast cancer ceils. MTT color-imetry was used to examine the effect of PGL3-DF3-DTA on growth of human breast cancer cells. By experiment on nude mice to observe the killing effect of PGL3-DF3-DTA on human breast cancer ceils. Results： Recombinant expression vector PGL3-DF3- DTA was highly expressed in human breast cancer cell line of DF3 positive, and it could kill the human breast cancer cells not only in vitro but also in vivo. Conclusion： Recombinant expression vector PGL3-DF3-DTA could produce specific lethal effect on human breast cancer cell line of DF3 positive.