小鼠毛囊周期中表皮朗格汉斯细胞及树突状表皮T细胞的变化

Changes of Langerhans cells (LC) and dendritic epidermal T cells during murine hair cycle

目的：观察拔毛诱导的小鼠毛发周期中毛囊间表皮LC及DETC的密度及形态变化，探讨二者与毛囊周期的关系。方法：选用自然休止期C57BL／6小鼠，拔毛诱导毛发进入生长期，应用ABC免疫组化法连续观察毛囊间表皮LC及DETC的密度及形态变化。结果：（1）密度变化：拔毛后1天LC及DETC与拔毛前无明显变化。拔毛后第2天开始二者密度较拔毛前升高（P〈0．05），拔毛后第4～8天二者密度最高（P〈0.01），以后逐渐下降，至拔毛后第17～20天二者密度基本恢复到拔毛前数值。（2）形态变化：拔毛前后2天内，多数LC及DETC胞体小，树突短小不明显；第4～8天，二者多数细胞体大，树突多且粗大，分枝明显；9～16天，胞体大小不一，树突变细；17～20天，胞体较小，树突短小。结论：拔毛诱导的小鼠毛发周期中毛囊间表皮LC及DETC的密度及形态变化与毛囊周期具有相关性。这种变化在皮肤免疫应答中可能起重要作用.

Objective： To observe the dynamic changes in the density and morphology of Langerhans cells （LC） and dendritic epidermal T cells （DETC） in murine inter- follicular epidermis during hair follicle cycle. Methods： C57BL/6 mice, all in natural telogen stage, were studied by ABC immunohistochemical technique for LC and DETC in murine inter- follicular epidermis during hair cycle in anagen stage induced by depilation. Results： The density of LC and DETC started to rise from day 2 （ P 〈 0.05） and reached the highest value on day 4 - 8 （ P 〈 0.01 ）. The density of LC and DETC resumed the normal value on day 17- 20（ P 〉 0.05）. Most LC and DETC were small and round with 1 - 2 short dendrites within 2 days after and before depilation. On day 4 - 8 both types of cells were big with 3 - 5 long dendritic processes and further divaricating. The cells resumed morphology of small bodies and 2 - 4 thin dendritic processes on day 17 - 20. Conclusion： The results clearly demonstrate that the dynamic changes of the density and morphology of LC and DETC in C57BL/6 mice inter - follicular epidermis are correlated with the hair cycle.