十二烷基苯磺酸钠对Wistar大鼠肝损害及血清酶逆向变化的影响

Effect of sodium dodecyl sulfate on hepatotoxicity and reverse change of enzymes in serum in Wistar rats

目的:探讨十二烷基苯磺酸钠(SDS)对Wistar大鼠肝脏的影响.方法:在饮水中加入SDS,使其终浓度分别为1000g/L,100g/L,10g/L,0g/L,持续让动物自由饮用7wk.实验结束后,处死动物,测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、乳酸脱氢酶(LDH)、碱性磷酸酶(ALP)和γ-谷氨酰转肽酶活性(γ-GT),并作肝组织病理形态学检查.结果:100g/LSDS剂量组的ALT,AST,γ-GT与LDH活性升高(与对照组比较,P<0.05),而1000g/LSDS剂量组的酶活性减低(与100g/L剂量组比较,P<0.01);各实验组的ALP活性与对照组比较,差异无统计学意义(P>0.05).肝组织病理检查显示,10g/LSDS剂量组肝组织病理形态学变化与对照组相似,100g/LSDS剂量组肝细胞轻度水肿、脂肪变性,1000g/LSDS剂量组肝细胞中度水肿、脂肪变性,有散在灶性坏死.结论:长期饮用含SDS的水对肝脏会有一定的损害,并产生血清酶的逆向变化现象.

AIM： To study the possible hepatotoxicity of Wistar rats induced by sodium dodecyl sulfate（SDS）. METHODS： SDS was added into the drinking water for getting the final concentrations as 10013, 100, 10 and 0 g/L respectively. Then let the rats drink the contaminated water freely for 7 weeks. After the experiment, the rats were killed and ALT, AST, LDH, ALP and γ-GT in the serum were assayed, and then the hepatic cells of the rats were examined in the way of pathomorphology. RESULTS： The activities of AST, LDH, ALT and γ-GT in 100 g/L group increased （ P 〈 0.05 vs the control group） , and then decreased in 1000 g/L group （ P 〈 0.01 vs 100 g/L group ） ; the activity of ALP in the experimental groups made no statistical significance compared with that of the control group. The pathological examination showed that the pathologic change of the liver tissues in the 10 g/L group made no difference from that in the control group, and the hepatic cells suffered from mild edema, fatty degeneration in the 100 g/L group; while the hepatic cells suffered from moderate edema and fatty degeneration and sporadic necrosis lesions in the 1000 g/L group. CONCLUSION： Long-time drinking the water containing SDS will do harm to the liver and cause reverse change of enzymes in the serum.