p38丝裂素活化蛋白激酶介导低氧预处理诱导的内质网应激相关的心肌细胞保护

Hypoxic preconditioning induces endoplasmic reticulum stress-related cardioprotection mediated by p38 mitogen-activated protein kinase

钙网蛋白(calreticulin,CRT)和caspase-12是重要的内质网(endoplasmicreticulum,ER)应激分子,本实验在心肌细胞低氧/复氧(hypoxia/reoxygenation,H/R)模型上观察低氧预处理(hypoxicpreconditioning,HPC)对CRT和caspase-12表达及活化的影响,探讨内质网应激(endoplasmicreticulumstress,ERS)在HPC保护机制中的意义及其细胞信号转导机制。原代培养的Sprague-Dawley乳鼠心肌细胞随机分为6组:H/R组、HPC+H/R组、SB203580+HPC+H/R组、SP600125+HPC+H/R组、HPC组和对照组。以细胞存活率、乳酸脱氢酶(lactatedehydrogenase,LDH)活性及流式细胞术检测细胞损伤情况;West-ernblot方法检测CRT和caspase-12表达、活化及p38丝裂素活化蛋白激酶(mitogen-activatedproteinkinases,MAPK)、c-JunN-terminalkinase(JNK)磷酸化水平。结果表明:(1)HPC具有细胞保护作用,与H/R组比较,HPC+H/R组细胞凋亡率和LDH漏出分别降低6.6%和70.0%,存活率增高6.4%;HPC前以特异性p38MAPK抑制剂SB203580预孵育消除HPC的保护作用,与HPC+H/R组相比,细胞凋亡率和LDH漏出分别增高5.4%和2.1倍,存活率降低5.4%,JNK特异性抑制剂SP600125预孵育对HPC的保护作用无明显影响。(2)H/R明显上调CRT表达(较对照组高8.1倍)和caspase-12活性(较对照组高33.2倍);单独HPC可诱导CRT表达增多(较对照组高2.6倍),但上调程度较H/R组低60%。H/R前进行HPC降低CRT过表达程度(降低72.4%)及caspase-12活化水平(降低59.6%)。(3)HPC前应用p38MAPK抑制剂,抑制CRT表达上调(分别较HPC+H/R组和HPC组低63.9%和71.9%),并消除HPC减轻H/R上调caspase-12活性的作用(较HPC+H/R组高7.1倍);HPC前抑制JNK活性对CRT、caspase-12表达和活化均无明显影响。上述结果提示:HPC可激发适当的ERS,抑制H/R诱导的过度ERS,减少ER凋亡信号介导的细胞凋亡。p38MAPK信号途径在HPC诱导的ER应激分子表达、抑制ER凋亡信号分子活化等机制中发挥重要作用。

Calreticulin （CRT）, an important Ca^2＋-binding molecular chaperone in the endoplasmic reticulum （ER）, and caspase- 12, a pivotal molecule mediating ER-initiated apoptosis, are involved in the ER stress （ERS）. Using primary cultured neonatal cardiomyocytes, CRT and caspase-12 expression and activation during hypoxic preconditioning （HPC） and hypoxia/reoxygenation （H/R） were studied to explore the role of ERS in cardioprotection by HPC. And by using SB203580 and SP600125 [the specific inhibitors of p38 mitogenactivated protein kinase （MAPK） and c-Jun N-terminal kinase （JNK） ] separately, the role of p38 MAPK in HPC-induced ERS was also detected. Neonatal cardiomyocytes were prepared from Sprague-Dawley rats aged 24 h, and cultured in DMEM medium containing 10% fetal bovine serum, and then randomly divided into six groups as follows： H/R, HPC＋H/R, SB203580＋HPC＋H/R,SP600125＋HPC＋H/R, HPC and control groups. H/R was produced by 2-hour hypoxia/14-hour reoxygenation, and HPC by 20-minute hypoxia/24-hour reoxygenation. Morphological studies, estimation of lactate dehydrogenase （LDH） leakage and flow cytometry were employed to assess cell apoptosis and necrosis. CRT and caspase-12 expression and activation, levels of phospho-p38 MAPK and phospho-JNK were detected by Western blot. All experiments were repeated at least four separate times. The results obtained are as follows： （1） HPC relieved the cell injury caused by H/R. Compared with that in H/R group, cells＇ survival rate in HPC＋H/R group increased by 6.4%, and the apoptosis rate and LDH leakage in the cell culture medium decreased by 6.6% and 70.0%, respectively. （2） H/R induced caspase-12 activation （33.2-fold increase in comparison with control） and CRT expression （8.1-fold increase in comparison with control）. HPC itself resulted in mild CRT up-regulation （2.6-fold increase in comparison with control）, but the extent of up-regulation was lower than that induced by H/R. HPC before H/R was found to relieve the over-expression of CRT induced by H/R （72.4% decrease）, and to inhibit the activation of caspase-12 （59.6% decrease）. （3） The protection of HPC and HPC-induced up-expression of CRT and inhibition of caspase-12 activation were almost eliminated when the inhibitor of p38 MAPK, not of JNK, was present before HPC. These results suggest that HPC protects the neonatal cardiomyocytes from severe ERS-induced apoptosis during sustained H/R through pre-invoking proper ERS response. Mild up-expression of CRT and inhibition of caspase-12 activation induced by HPC, which are important protection factors, are mediated by p38 MAPK, not by JNK.