改良“二袖套管法”大鼠原位肝移植模型的建立

Two-cuff technique was applied in the establishment of orthotopic liver transplantation in rats

目的：建立稳定的大鼠原位肝移植模型，为进一步研究核苷酸圈套技术抗大鼠肝移植急性排斥建立良好的技术平台。方法：实验以SD大鼠为模型动物，采用改良“二袖套管法”进行原位肝移植实验，统计受体大鼠术后生存时间，并常规病理检测术后移植肝病理变化。结果：共进行大鼠原位肝移植实验82次。实验后期能稳定建立起原位肝移植模型，供体手术时间25～35min；供肝冷缺血时间40～55min；受体无肝期时间13～24min；受体手术时间45～65min；2周生存率为93％。移植肝组织在术后1周可见大量的单核淋巴细胞浸润，在门静脉周围的汇管区尤为明显；肝脏实质有点状坏死。术后2周细胞浸润增加，肝实质可见桥状坏死。术后1月移植肝组织内细胞浸润和坏死减少，且可见肝细胞和胆管增殖。结论：采用改良“二袖套管法”建立大鼠原位肝移植模型手术成功率高，稳定性好，是研究肝移植术后机体生化生理改变、免疫状态变化等的良好技术平台。

Objective： To establishment an ideal model for orthotopic liver transplantation. Methods： SD rats were used as experimental animals. Two-cuff（ portal vein and infrahepatic vena cava）technique was applied in the establishment of orthotopic liver transplantation in rats. Survival time of recipient rats were analyzed and morphological investigations were studied on the grafts after liver transplantation. Results： With the modification, a successful rate of 100% was achieved in the production of the experimental model. Donor operating time was 25 - 35 min; the grafts cold ischemic time was 40 - 55 min; recipients operating time was 45 - 65 min with anhepatic period of 13 - 24 min. Two week survival rate of recipients was 93%. Hepatocellular necrosis was observed on day 7 - 14 after liver transplantation and a large numbers of mononuclear cells infiltrating into the portal areas. On day 30 after liver transplantation, the parenchyma cells showed a nearly normal appearance, bile duct proliferation was seen in portal areas, and a few diploid hepatocytes were found. Conclusion： The liver transplantation model using two-cuff technique is easily available and highly reproducible. This model can be used in experimental study on physiological and immunological change after liver transplantation.