榄香烯乳对胃癌细胞人端粒酶反转录酶及其启动子的影响

Effect of elemen on promoter and human telomerase reverse transcriptase(hTERT) of gastric cancer cell

目的:观察榄香烯乳对胃癌细胞株SGC7901人端粒酶反转录酶(hTERT)表达及其启动子的影响,以深入了解榄香烯乳的抗癌机制。方法:以榄香烯乳处理胃癌细胞,分别采用实时定量PCR和免疫荧光标记法检测癌细胞hTERT mRNA和蛋白水平的表达。采用脂质体转染法将携带hTERT启动子和报告基因的质粒转染至胃癌细胞株SGC7901细胞中,2 h后加入不同浓度的榄香烯乳,孵育48 h后,分别检测其报告基因萤虫素酶活性。结果:榄香烯乳处理后,胃癌SGC7901细胞hTERT mRNA和蛋白水平呈浓度和时间依赖性下调。榄香烯乳处理组萤虫素酶活性下降,且呈浓度依赖性。结论:榄香烯乳可能通过下调hTERT启动子活性,抑制hTERT表达,从而抑制胃癌细胞端粒酶活性。

Objective： To investigate the effect of elemen on expression and promoter of human telomerase reverse transcriptase（hTERT） in gastric cancer cell. Methods： After gastric cancer cell line SGC7901 were treated with different dose of elemen, mRNA and protein of hTERT were determined by real-time PCR and immunofluorescense labeling assay, respectively. The plasmid inserted hTERT promoter was transfected into SGC7901 cells by oligofecamine, and different doses of elemen were added into culture media 2 hours later. After 48 hours, luciferase activity of gastric cancer cell was determined. Results： The expression of mRNA and protein of hTERT of cells treated with elemen were down-regulated in dose-and time-dependent manner. The luciferase activity of cancer cells treated with elemen were inhibited in dose-dependent manner. Conclusion： Elemene may suppress telomerase activity through inhibiting expression of hTERT promoter of gastric cancer cell.