硒和维生素E对丙烯酰胺致V79细胞毒性的拮抗作用

Antagonistic Effect of Selenium and Vitamin E on Acrylamide Toxicity

目的研究不同浓度的维生素E和（或）硒对丙烯酰胺（AA）染毒V79细胞的拮抗作用。方法利用细胞毒性实验（MTT法）计算细胞相对存活率，用单细胞凝胶电泳检测DNA损伤，并对实验数据进行统计学分析。结果0．5～10．0μmol／L的硒和（或）2．5-40．0mmol／L维生素E与1．5mmol／L的丙烯酰胺作用于体外培养的V79细胞，实验结果表明，低浓度的硒与维生素E均可降低丙烯酰胺所致的细胞毒性和基因毒性，0．75mmol／L的AA已显示明显的细胞毒性，V79细胞的相对存活率（77．36％）明显低于阴性对照组，AA对V79细胞的毒性作用有明显的剂量-反应关系。高浓度的硒则起相反的作用；硒和维生素E共同作用，其拮抗效果明显优于硒、维生素E单独作用。结论在体外细胞培养条件下，一定浓度的维生素E和硒对AA染毒V79细胞的细胞毒性、遗传毒性有拮抗作用，且具有协同效应。

[ Objective] To study the antagonistic effect of selenium and vitamin E on acrylamide toxicity in V79 cells. [ Methods] MTF method was applied to count the cell viability; single cell gel electrophoresis was used to detect the DNA damages; statistic analysis was made on the experimental data. [ Results ] Selenium （ 0.5 ± 10.0 μmol/L ） and （ or ） vitamin E （ 2.5 ± 40.0 mmol/L } could reduce the cyctoxicity and mutagenicity caused by acrylamide in fhe cultured V79 cells ; 0.75mmol/L of the AA indicated obvi- ous toxicity; the relative livability of V79 cells （77.36%） was obviously lower than that of the control group; AA had obvious dose reaction relationship on the toxicity of V79 cells; the synergistic effect of selenium combined with vitamin E produced antagonistic effect that obviously excelled that of the individual selenium or vitamin E. [ Conclusion] Under the condition of in vitro cells culture, the combination of selenium or vitamin E had antagonistic effect on the cyctoxicity and mutagenicity of V79 cells, meanwhile, with synergistic effect.