摘要
目的:建立HPLC法测定复方硫酸新霉素滴眼液中地塞米松磷酸钠的含量。方法:采用Eclipse XDB-C18色谱柱(250 mm×4．6mm,5μm),流动相为0．75％三乙胺溶液-甲醇-乙腈(40:55:5)流速1．0 ml·min-1,检测波长为242 nm。结果:地塞米松磷酸钠在0．1～1．21μg的范围内线性关系良好(r=0．999 8),平均回收率为99．0％,RSD为1．6％。结论:该法简便、快捷、准确,能更好的控制其质量。
Objective： To establish a HPLC method for Determination the content of Dexamethasone Sodium Phosphate in Compound Neomycin Sulfate Eye Drops. Method： Ecipse XDB-C18 column（250 mm×4.6 mm,5 μm）was used, 0. 75% Triethylamine solution-methanol-acetonitrile（40： 55：5 ） as mobile phase the flow rate was 1.0 ml·min^-1 and detection wavelength was 242 nm. Result： The linear range of Dexamethasone Sodium Phosphate was 0.1-1.21 μg（ r = 0. 999 8 ,n = 5 ）. The average recovery was 99.0% with RSD 1.6%. Conclusion： The method is simole, raoid and accurate. It can be used for aualitv control of this oreoaration.
出处
《中国药师》
CAS
2006年第11期1013-1014,共2页
China Pharmacist
