6种桐籽粕提取物对鸡新城疫病毒体内体外抗病毒作用的研究

Study On Anti-NDV Capacity of Six Different Tung-seed Meal Extracts in Vivo and in Vitro

为了解6种桐籽粕提取物对正常单层鸡胚成纤维细胞(CEF)的毒性作用,以及对鸡新城疫病毒(NDV)体内外抗病毒的作用效果,首先将桐籽粕提取物直接作用于正常的单层CEF,然后将桐籽粕提取物与鸡新城疫病毒37℃作用2 h,分别接种10日龄鸡胚和加入单层CEF。试验结果表明:过NKA 70%醇洗部分,对正常CEF的毒性作用最大,水提物毒性作用最小。而对新城疫病毒,水提物(0.125 m g/m l)的抗病毒效果最好;其次为过NKA大孔吸附树脂30%醇洗部分(0.2 m g/m l),然后依次是水提物(0.5 m g/m l)、水提物(0.25 m g/m l)、无水乙醇析出物(0.2 m g/m l)。

To investigate the cytotoxical effect of six extracts from Tung-seed meal on chick embryo fibroblast （CEF） and to determine the effect of extracts on new castle disease virus （NDV）in vivo and in vitro. First, Tung-seed meal extracts were treated with NDV for 2 h at 37 C, and then inoculated in 10-day CEF. The results showed that extract with 70% ethanol via NKA Resin has the most cytotoxicity, while extract with water has the least. The extract with water was most effective in anti-NDV （0. 125 mg/ml）, the anti-NDV capacity of extract with 30% ethanol extract through NKA Resin （0.2 mg/ml）, water extract（0.5 mg/ml, 0.25 mg/ml）, absolute alcohol extract （0.2mg/ml） were decreased in order.