三七总皂苷壳聚糖缓释微球的制备及体外释放特性研究

Preparation and in vitro characterization of saponins of Panax notoginseng-chitosan microsphere

目的制备三七总皂苷壳聚糖缓释微球,并对其体外释药特性进行研究。方法采用乳化交联法制备三七总皂苷壳聚糖微球,以粒径分布、包封率、载药量及体外释药速度为评价指标,考察处方因素对壳聚糖微球的影响。并采用正交设计L9(34)对处方进行优化。结果微球的平均粒径为(4.2±0.3)μm,包封率为(28.58±2.76)%(n=3),载药量为(17.15±1.65)%(n=3)。结论通过优化处方和制备工艺,采用乳化交联法制备的三七总皂苷壳聚糖缓释微球,其体外释药具有明显的缓释作用且制备工艺简单。

Objective To prepare and evaluate saponins of Panax notoginseng-chitosan microspheres with sustained-release property. Methods The microspheres were prepared by water/oil emulsion polymerization and by cross-linking with glutaraldehyde. The effects of preparation compositions on particle size, particle morphology, encapsulation efficacy and drug release rate in vitro were investigated by orthogonal design L9 （ 34 ）. Results Mean particle size was （4.2 ± 0.3 ）μm, encapsulation efficacy was （ 28.58 ± 2.76） % （ n = 3 ）, loading efficiency was （ 17.15 ± 1.65 ） % （ n = 3 ）. Conclusion Saponins-chitosan microspheres with sustained -release property could be prepared effectively.