高效液相色谱法测定细辛脑注射液的含量及有关物质

Analysis of asarone and impurities in asarone injection by HPLC

目的建立测定细辛脑注射液的含量及有关物质的高效液相色谱法。方法采用Kromasil C18柱，以甲醇-水（体积比80：20）为流动相，检测波长313nm。结果本色谱条件能分离细辛脑及有关物质；在2～80mg·L^-1浓度范围内，细辛脑的峰面积与其浓度呈线性关系，方法平均回收率为100．2％，RSD为0．37％。结论本法可用于细辛脑注射液的含量及有关物质测定。

Objective To establish an HPLC method for the determination of asarone and impurities in asarone injection. Methods The analysis was performed on a Kromasil-C18 column using methanol-water（ 80： 20）as mobile phase and the detection wavelength was 313 nm. Results Asarone and impurities could be separated well under the condition. Linear calibration curve was obtained in the range of 2 -80 mg·L^-1 with an average recovery of 100.2% （RSD = 0.37% ）. Conclusion The method can be used for the quality control of asarone injection.