高效液相色谱法同时测定感冒清胶囊中两组分的含量

Simultaneous determination of moroxydine hydrochloride and paracetamol in Ganmaoqing capsules by HPLC

目的建立测定感冒清胶囊中盐酸吗啉胍和对乙酰氨基酚含量的高效液相色谱法。方法采用高效液相色谱，以C18为色谱柱，以甲醇-水-冰乙酸-三乙胺（体积比250：750：3，75：0．75）为流动相，流速为1．0mL／min，检测波长为249nm。结果盐酸吗啉胍和对乙酰氨基酚分别在0．086～0.301mg／mL和0．082～0．298mg／mL浓度范围内呈现良好的线性关系（r=0．9999）。盐酸吗啉胍和对乙酰氨基酚平均加样回收率分别为100．9％（n=9，RSD=2．3％）和99．6％（n=9，RSD=1.8％）。结论本法操作简便，灵敏度高，重复性好，测定结果准确，可用于感冒清胶囊的质量控制。

Objective To establish an HPLC method for simultaneous determination of moroxydine hydroehloride and paracetamol in Ganmaoqing capsules. Methods The C18 column（4.6 mm× 250 mm,5 μm）was used with methanol-water-acetic acid-triethylamine buffer （250：750： 3.75：0.75 ） ） as mobile phase. The flow rate was 1.0 mL/min and the detection wavelength 249 nm. Results The calibration curves were linear over the range of 0. 086 - 0.301 mg/ml （ r = 0. 9999 ） for moroxydine hydrochloride and 0. 082- 0. 298 mg/mL（ r = 0. 9999 ）for paracetamol. The average recoveries of were 100.9% （RSD = 2. 3 % ,n = 9 ）for moroxydine hydrochloride and 99.6% （RSD = 1.8% ,n =9） for paracetamol. Conclusion The method is simple, sensitive and accurate,which could be used for the quality control of Ganmaoqing capsules.