从人血凝块中提取基因组DNA

A method for DNA extraction from human blood clots

目的从人血凝块中提取基因组DNA。方法室温自然解冻,机械磨碎,高盐、高EDTA提取液处理,消化液消化,酚氯仿抽提。结果用改良酚氯仿法从人血凝块中成功提取基因组DNA,提取的DNA浓度为:(32±10)mg/L,且光吸收比值A260/A280>1.8。结论用改良法提取的基因组DNA的总量较高,提取效率高,PCR扩增效果好,可很好的用于血凝块基因组DNA的提取。

Objective To explore a simple method of DNA extraction from human blood clots. Methods Blodd clots thawed in rcom temperature, homogenized extraction buffer including higher sodium chloride and higher EDTA, the homogenized samples were digested with digestion buffer and extracted with phenol-chloroform. Results The genomie DNA were successfully extracted. The average quantity of the extracted DNA was （ 32 ± 10 ） mg/L, while A260/A280 〉 1.8. Conclusion The improved extraction method was reliable for obtaining high quantities of DNA from blood clot suited for PCR amplification.