几个因素对草地早熟禾种子愈伤组织诱导的影响

EFFECTS OF SEVERAL FACTORS ON CALLUS INDUCTION FROM SEEDS OF POA PRATENSIS L.

对草地早熟禾种子分别在每天12 h的光照和完全黑暗以及不同蔗糖浓度、不同接种季节条件下愈伤组织的诱导进行了研究,结果表明:在暗培养条件下,早熟禾种子愈伤组织诱导率为55.6%,而在每天12 h光照条件下则为38.1%;蔗糖浓度以30,15 g/L为好,其愈伤组织诱导率分别是62.5%,58.3%;培养季节春、夏、秋都比较适宜,冬季不适合早熟禾种子愈伤组织的诱导。

Seeds of Poa pratensis L. were cultured under 12 h L ＋ with different sucrose concentrations to induce callus differentiation. 12 h D photoperiod in different seasons in MS medium supplemented Culture in the dark gave higher induction rate （55.6%） than 12 h L ＋ 12 h D photoperiod （38. 1% ）. A sucrose concentration of 30 or 15 g/L gave satisfactory results, the callus induction rate being 62.5% and 58.3% , respectively. Culture in spring, summer or autumn was suitable while culture in winter was not.