植物激素对大蒜茎盘组织培养的影响

EFFECTS OF PHYTOHORMONES ON CALLUS INDUCTION SHOOT DIFFERENTIATION AND GENETIC VARIATION IN THE TISSUE CULTURE OF STEM DISCS OF GARLIC(ALLIUM SATIVUM L.)

大蒜愈伤组织在培养过程中容易发生变异,因此可以有目的的利用这些体细胞变异创造新的种质资源。结果表明:高浓度的生长素有利于大蒜愈伤组织的诱导,但并不利于其分化。MS+NAA 2 mg/L+BA 1 mg/L是适宜于愈伤组织增殖和分化的培养基。继代2次的愈伤组织芽分化能力最强,但继代6次后,试管苗根尖染色体的变异率达到55.5%,这种变异可能与有丝分裂异常有关。

Variations tend to occur during tissue cuhure of garlic （Allium sativum L. ） and, therefore, these somatic variations can be exploited to create novel germplasms. In the present study, the stem disks of garlic were used as explants. Higher auxin concentrations appeared favorable for callus induction but not for callus differentiation. MS ＋ NAA 2 mg/L ＋ BA 1 mg/L was suitable for callus proliferation and differentiation. Calli subcultured twice showed the highest rate of shoot differentiation. The chromosomes of the root tips showed a variation rate of 55.5% when subcultured 6 times. These variations are presumed to be associated with abnormal mitosis.