ZY-800KB型KB法抑菌圈测量仪临床应用评价

Clinical evaluation of ZY-800KB inhibition zone measures

目的评价ZY-800KB型KB法抑菌圈测量仪（智能药敏鉴定分析系统）临床应用价值。方法采用美国临床实验室标准化研究所（CLSI／原名NCCLS）推荐的纸片扩散法进行抗生素敏感性试验。用游标卡尺和抑菌圈测量仪分别测量抑菌环直径，比较两者测量结果一致性及时耗。结果手工法和仪器法对809株常见细菌K—B法体外药敏试验抑菌环直径进行了8889对测试，两者报告的药敏结果一致性达99.9％（8881／8889），所出现的8例误差均为次要误差。手工法和仪器法的质控菌抑菌环直径测定值具有高度的一致性，95．8％的测试对差值≤1mm，最大差值在2mm以内。仪器法抑菌环直径测量、签发报告和录入WHONET数据库所需时间约为手工法的（1／31／4）。结论ZY-800KB型KB法抑菌圈测量仪与游标卡尺手工测量法的抑菌环直径测量结果具有很好的一致性，并明显提高工作效率。

Objective To evaluate the clinical value of ZY-800KB mode inhibition zone measures. Methods Antimicrobial susceptibility test was detected by disk-diffusion method recommended by CLSI/NCCLS, inhibition zones were measured by inhibition zone measures and vernier caliper respectively. The consistency of antimicrobial susceptibility test results and time consumption of two methods were compared. Results A total of 8889 inhibition zones of 809 stains common pathogenic bacteria were measured by inhibition zone measures and vernier caliper at the same time, the con- sistency of antimicrobial susceptibility test results reported by two methods was 99. 9% （8881/8889）, only 8 cases minor error occurred. There was a good consistency between common antibiotics＇ inhibition zone diameter values of quality control strains measured by inhibition zone measures and that of vernier caliper, the difference value of 95.8% results were no more than lmm, and the maximum difference value was no more than 2mm. Less time was cost in the courses of inhibition zone measuring, report issuing and logging in WHO NET database by inhibition zone measures than handiwork. Conclusions ZY-800KB inhibition zone measures have a good consistency with vernier caliper in inhibition zone measuring, and it can enhance work efficiency remarkably.