受体血预先经门静脉输入供体延长肝移植大鼠生存时间

Prolonged survival of rat hepatic allograft after pretransplant intraportal infusion of recipient blood to the donor

目的:探讨受体全血预先经门静脉输入供体对大鼠肝移植排斥反应的作用．方法:采用异系大鼠肝移植模型,供体为ACI大鼠．受体为LEW大鼠．将实验组受体大鼠全血1 mL经门静脉输入供体肝内,7 d后将处理后的供体肝脏移植于受体．经门静脉将生理盐水1 mL输入对照组供体肝内,7 d后将此供体肝脏移植于受体．观察大鼠生存时间,分期分批处死大鼠,从移植肝及受体脾分离T淋巴细胞,测定移植肝及受体脾内中供体来源活化T淋巴细胞的比例,检测细胞因子mRNA的表达．结果:肝移植大鼠生存时间:实验组31．7±7．6 d,对照组11．1±2．1 d．两组差异显著(P< 0．05)．移植后7 d从移植肝分离的浸润T淋巴细胞分析结果:实验组OX76+CD4+细胞25．6%．OX76+CD8+细胞26．6％;对照组OX76+CD4+细胞6．2％,OX76+CD8+细胞7．4％．实验组移植肝内有大量供体来源的活化T淋巴细胞．实验组肝内OX76+CD8+细胞可见IL-10 mRNA及IFN-γmRNA的表达．结论:受体血预先经门静脉输入供体,可以延长异系大鼠肝移植生存时间．有限的移植物抗宿主反应,可能是这种肝移植排斥反应抑制的机制．

AIM： To investigate the effect of pretransplant intraportal administration of recipient blood into the donor on the survival of rat hepatic allograft. METHODS： Male LEW （RT1） and ACI （RT1a） rats were used as liver transplant recipients and donors, respectively. In experimental group, 7 d prior to transplantation, ACI donors were anesthetized with ether and transfused with freshly heparinized LEW recipient blood （1 mL） via the portal vein. In control group, the rats only received 1 mL normal saline 7 d before transplantation. The serum levels of interferon-γ （IFN-γ） were determined after transplantation. Immunocompetent cells from the donors were identified with anti-donor class Ⅱ MHC （RTIBa） （OX-76） antibodies. The number of donor-derived CD4^＋ and CD8^＋ T cells in hepatic allograft or recipient spleen was examined. Reverse transcriptionpolymerase chain reaction was used to detect the mRNA expression of cytokines in the allograft. RESULTS： LEW rats transplanted with hepatic allograft from ACI rats survived a mean of 11.1± 2.1 days. Intraportal preadministration of recipient LEW blood significantly increased the survival of LEW recipients with ACI livers to 31.7 ± 7.6 days （P 〈 0.05）. The serum level of IFN-T was significantly increased in the experimental group as compared with that in the control group. Cellular infiltration of the hepatic allograft in experimental group was significantly lower than that in the control group. The ratio of OX76^＋CD4^＋ or OX76^＋CD8^＋ T cells to CD4^＋ or CD8^＋ cells was significantly greater in hepatic allograft pretreated with LEW recipient blood than that in the controls （OX76^＋CD4^＋ cells： 25.6% vs 6.2%, P 〈 0.05; OX76^＋CD8^＋ cells： 26.6% vs 7.4%, P 〈 0.05）, but not in recipient spleen. OX76^＋CD8^＋ T cells from hepatic allograft in the experimental group expressed IFN-γ and interleukin-10 mRNA, but not interleukin-2 mRNA. CONCLUSION： Pretransplant intraportal infusion of recipient blood to the donor rats prolonged the survival of hepatic allograft, which may be associated with the regional graft-versus-host reaction.