DNA修复基因XRCC1多态与食管癌TNM分期及区域淋巴结转移的相关性研究

The relationship between polymorphism in XRCC1 and TNM staging and lymph node metastasis of esophageal squamous cell carcinoma

目的研究DNA修复基因XRCC1Arg194Trp和Arg399Gln多态与食管鳞状细胞癌临床病理TNM分期及区域淋巴结转移的相关性,探讨其对食管癌临床表型的影响。方法以PCR和PCR-RFLP方法分析了182例食管癌病人的上述基因多态,比较不同TNM分期及区域淋巴结转移的食管癌病人各基因型频率分布的差异。结果食管癌IIb～IV期及有区域淋巴结转移病人XRCC1Arg194Trp基因型及Arg399Gln基因型频率与0～IIa期及无区域淋巴结转移病人虽有差别,但无统计学意义。食管癌区域淋巴结转移病人携带XRCC1194Arg/Trp杂合型和399Arg/Arg野生型基因型频率为32.3%,显著高于携带XRCC1194Arg/Arg基因型的20.0%(P=0.01)。结论DNA修复基因XRCC1多态中携带194变异型基因型和399野生型基因型的病人区域淋巴结转移率较高,病变进展较快,因此与食管癌TNM分期和区域淋巴结转移存在相关型,两者对食管癌病人的预后有影响。

Objective To agsess the relationship between polymaorphism of XRCC1 Arg194Trp Arg399Gtn and ESCC TNM stage and regional lymph node metastasis. Methods PCR and PCR-RFLP were used to detect polymorphism of XRCC1 Arg194Trp and Arg399Gin. The results were analyzed and compared to TNM staging and regional lymph node metastasis, Results The XRCC1 194Arg/ Trp and 399Arg/Arg wildtype in ESCC with metastasis is 32.3 %, which is higher than 194Arg/Arg （20.0%, P = 0.01 ）. There was no remarkable difference between Ilb-Ⅳ stage ESCC with lymph node metastasis and 0-11a stage without metastasis. Conclusion The ESCC patients with 194 variability and 399 wildtype had high metastasis rate, XRCC1 Arg194Trp and Arg399Gtn had relationship with ESCC TNM stage and lymph node metastasis. Both of them affected to pragnosis of ESCC.