免疫比浊法和ELISA法测定类风湿因子的比较

Investigation of clinical significance of rheumatoid factor determination with different methods

目的探讨不同方法测定类风湿因子(RF)的临床应用价值。方法采用免疫速率散射比浊法和ELISA法检测类风湿关节炎患者38例,非类风湿关节炎患者73例及健康体检者20例的血清类风湿因子。结果免疫比浊法检测类风湿关节炎患者、非类风湿关节炎患者的RF阳性率分别为50%、21.9%,20例健康者无阳性结果,此法的诊断效率为73.3%;ELISA法类检测RF阳性率分别为76.3%、41.1%和5%,其诊断效率为69.5%。结论ELISA法检测类风湿因子的敏感性较免疫比浊法高,但特异性相对较低,诊断效率略低于免疫比浊法。

Objective To investigate the significance of rheumatoid factor determination with different methods. Methods The concentrations of rheumatoid factor in serum were detected with immunoturibidimetric assay and ELISA in 38 cases of rheumatoid arthritis, 73 cases of other diseases without rheumatical arthritis and 20 healthy controls. Results The positive percent of group with rheumatoid arthritis, group with other diseases and controls were 50%, 21. 9% and 0, respectively with immunoturibidimetric assay; and 76.3%, 41.1% and 5%, respectively with ELISA. The diagnostic efficiency using immunoturibidimetric assay and EI.ISA were 73.3% and 69.5%, respectively. Conclusion Rheumatoid factor determination with ELISA is more sensitive than that with immunoturibidimetric assay for the diagnostics of rheumatoid arthritis. But the determination with immunoturibidimetrie assay is more specific to rheumatoid arthritis than that with ELISA. The diagnostic efficiency using immunoturibidimetric assay is little higher than that using ELISA.