livin蛋白和突变型p53蛋白在非小细胞肺癌中的表达及相关性研究——附40例分析

Study on the expression and correlation of livin and mutant p53 proteins in non-small cell lung cancer

目的:探讨非小细胞肺癌(non-small cell lungcancer,NSCLC)肺组织的livin蛋白和突变型p53蛋白的表达水平,了解2种蛋白与临床特征的关系。方法:采用免疫组织化学(免疫组化)法分别检测40例NSCLC患者的病变肺组织(肺癌组)以及12例正常或肺良性疾病患者肺组织(对照组)的livin蛋白和突变型p53蛋白的表达水平,并分析2者与肺癌组的组织类型、分化程度、淋巴结转移之间的关系,以及分析2种蛋白表达的相关性。结果:肺癌组的livin蛋白和突变型p53蛋白阳性表达率均高于对照组(均为P<0·01)。在NSCLC组中,与无淋巴结转移的肺组织比较,有淋巴结转移的肺组织中livin蛋白和突变型p53蛋白的阳性表达率较高(73%比17%,64%比27%,均为P<0·01)。肺癌组中不同分化程度肺组织的livin蛋白和突变型p53蛋白的阳性表达率差异无统计学意义。livin蛋白与突变型p53蛋白的表达无相关关系。结论:livin蛋白与突变型p53蛋白可作为诊断NSCLC的特异性指标,亦为抗肿瘤基因治疗提供了新的靶点。

Objective： To study the expression of the livin and mutant p53 protein in lung tissues of non-small cell lung cancer （NSCLC） and their relationship with the clinical characteristic. Methods： The expression of livin and mutant p53 protein was detected by immunohistochemistry method, in lung tissues of 40 patients with NSCLC and 12 normal control or patients with benign lung diseases Expression of these protein was analyzed for correlation with histological typing, degree of differentiation and lymph node involvement. Results： The positive rates of livin and mutant p53 in NSCLC group were significantly higher than those in normal and benign lung tissue. The positive rate of livin in NSCLC with and without lymph node involvement was 73% and 17% , respectively. The positive rate of mutant p53 in NSCLC with and without lymph node involvement was 64% and 27% respectively. There was no statisti- cally significant correlation between livin or p53 expression and histological degree. No eorrelation was found between p53 and livin protein expression. Conclusion： The expression of livin and p53 proteins can be used as characteristic parameters for the diagnosis of NSCLC. Livin and p53 can be considered as a target for anti-tumor gene therapy.