梗阻性黄疸大鼠小肠肠系膜微循环变化及意义

Changes and significance of small intestine mesenteric microcirculation in rats with obstructive jaundice

目的以肠系膜为微循环观测窗,探讨梗阻性黄疸(简称梗黄)对肠系膜微循环的影响。方法将60只SD大鼠均分为对照组和梗黄组,每组分1、3、7d三个观测时相。将大鼠腹腔内一段回肠袢肠系膜平铺于恒温灌流盒作为微循环观察窗,用微循环显微镜及视频图像分析系统观测肠系膜微循环的动态变化并记录结果。结果梗黄组大鼠肠系膜微循环从微血管形态、微血管流态及微血管周围状态三个方面均发生明显变化,主要表现为毛细血管与微静脉扩张,血流减慢,红细胞聚集,微栓形成、毛细血管通透性增加及出血等,与对照组各相同时相比较差异均有统计学意义(P＜0．05)。结论梗阻性黄疸可引起大鼠肠系膜微循环障碍。

Objective To explore how obstructive jaundice affects small mtestme mesentenc microclrculation by using mesentery as observing area. Methods Sixty SD rats were divided into two groups （control group and obstructive jaundice group） randomly and observed at 1st,3rd and 7th day. The ileocecal junction mesentery that was used as observing area was spread on the irrigating and flowing box, and its dynamic change was observed under the microcirculatory microscopy and images analyzing system, and the results were recorded. Results The alternation of mesenteric microcirculation was apparent in obstructive jaundice group,including the changes of microvascular morphology, microvascular flow state and microvascular peripheral state as follows：the dilation of capillary vessels and venules, the slow velocity of blood flow, the congregated erythroeytes, micro-thrombus formation ,the higher permeability of microvessels,hemorrhage. There was significant difference between control group and obstructive jaundice group （P〈 0.05）. Conclusion Obstructive jaundice could lead to the disturbance of rats＇ mesenteric microcirculation.