离心法定量检测尿沉渣准确性研究

Investigation on the Accuracy of Quantative Analysis of Urinary Sediment

目的:研究离心沉淀对尿沉渣有形成分定量检测准确性的影响和影响程度。方法:以红细胞尿液为标本,对高中低三种不同浓度红细胞的尿液分别进行离心和不离心沉淀的定量检测,检测结果用t检验统计分析。结果:高中低三种不同浓度红细胞离心和不离心沉淀的定量检测结果均具有显著差异P<0.01。结论:引起定量检测离心沉淀尿沉渣误差的因素很多,但有形成分不完全离心沉淀是主要因素,建议改进离心法定量检测尿沉渣的方法,保证检测结果的准确性。

Objective： To evaluate centrifugalization- precipitation method＇s effects on the accuracy of quantative analysis of the formed elements in the urinary sediments. Method： Testing erythocyturia containing high, medium, low concentration RBC with/without centrifugalization -precipitation method, using t - test to evaluate the results. Result： Test with/without centrifugalization - precipitation method, urine with high, medium, low concentration RBC has different result （ P 〈 0.01 ）. Conclusion： Among the factors that affect the test, incomplete centrifugalization of the formed elements is the most important. In order to guarantee the accuracy of the results, the centrifugalization- precipitation method for the quantative analysis of urine should be improved.