一种海马肠道疾病病原体的鉴定及其对抗生素的敏感性研究

Study on Isolation,Identification and Sensitivity to Antibioties of Seahorse H.Kelloggi

〔目的〕研究海马致病病原体,筛选不同用途海马的高效病害治疗物,促进我国海马养殖的发展。〔方法〕室外采集标本分离患病海马病原体,从患病海马体内分离到3株菌株,用分离菌株的纯培养物感染健康海马。〔结果〕48h内实验海马发病,其临床症状与原患病海马相似,经形态学、生化和室内感染试验鉴定为副溶血弧菌(Vibrio parahemolyticus)。药敏试验表明,所分离菌株对链霉素、利副平、卡那霉素、环丙沙星、氟派酸、四环素、复达欣、菌必治、奈定酸等抗菌药表现为高敏感性;对红霉素、新霉素、先锋霉素Ⅵ、强力霉素、新生霉素、头孢呋肟、呋喃妥因等药物敏感性较高;对氨苄青霉素、羟苄青霉素、头孢氨苄、林可霉素等药物敏感性较差。〔结论〕弧菌对海马的致病性作用很明显,但不同的情况下表现不同。不同的药物对弧菌的作用不同,而不同用途的海马其所用药物也不尽相同。

Objective To get a better understandings in pathogens of seahorse H.Kelloggi for screening effective medicine,and to faciliate the development of seahorse husbandary industry. Methods Three strains of bacteria were isolated from the diseased seahorse, which were cultured in tanks. The biochemical characteristics of these bacteria isolated were the same as those of Vibrio parahemolyticus and these bacteria isolated were identified as Vibrio parahemolyticus. Results Healthy seahorse ,which were injected with the bacterial isolation solution, were infected after 48 hours. These seahorses showed the similar characters as the illed seahorses did. Conclusion The bacterial isolates were highly sensitives to streptomycin, rifampin, kanamycin, ciprofloxacin, norfloxacin, tetracycline, of oxacincefiazidime, cefiriaxone, nalidixic acid etc.