肝硬变患者胃冠状静脉壁生长因子表达与分布的免疫组化分析

Immunohistochemical Analysis of Growth Factor Expression and Localization in Gastric Coronary Vein of Cirrhotic Patients

采用免疫组化技术观察肝硬变患者（ｎ＝３０）、非肝硬变患者（ｎ＝１０）的胃冠状静脉壁中血小板衍生生长因子、碱性成纤维细胞生长因子、表皮生长因子、转化生长因子α的表达与分布。发现肝硬变患者上述４项的强阳性率分别为９３％、８９％、７０％和６８％，而非肝硬变患者均为阴性。同时发现肝硬变患者胃冠状静脉有内皮细胞损伤、平滑肌细胞肥大增殖和血管构形改建。结果表明，肝硬变胃冠状静脉可产生生长因子。

This study was designed to investigate the expression and localization of PDGF, bFGF, EGF, and TGFα in gastric coronary vein of cirrhotic ( n =30) and non cirrhotic ( n =10) patients using immunohistochemistry technique. The strongly positive immunostaining rate were 93%, 89%, 70% and 68% respectively in cirrhotic patients. The immunostaining was negative in non cirrhotic patients. The damage to endothelium, hypertrophy and hyperplasia of vascular smooth muscle cells and vascular remodeling were seen in gastric coronary vein of cirrhotic patients. These results suggested that gastric coronary vein could produce growth factor during cirrhosis, the growth factor can act on the vascular function and/or structure via autocrine paracrine mechanism.