辽宁省主要玉米自交系的SSR遗传多样性分析

Genetic Diversity Revealed by SSR Among Maize Inbred Lines Used Predominantly in Liaoning Province

利用SSR标记研究了37份玉米自交系的遗传多样性,从85对SSR引物中筛选出70对扩增产物具有稳定多态性的引物。这70对引物在供试材料中检测出260个等位基因变异,每对引物检测等位基因2～7个,平均3.71个,每个位点的多态性信息量(PIC)变化为0.157～0.813,平均为0.564。37份自交系之间的遗传相似系数变化范围为0.69～0.89。UPGMA聚类分析结果表明,37份供试自交系划分为6个类群,分类结果与系谱来源基本一致。生产上主要推广杂交种的亲本大多来自不同的类群。

Simple sequence repeats（SSR） were used to detect genetic diversity among 37 maize（Zea mays L.） inbred lines, which were used widely in Liaoning province. Seventy SSR primers selected from 85 primers produced stable profiles amplified in sample of 37 inbred lines. Seventy primers produced 260 polymorphic amplified fragments. The average number of allele per SSR locus was 3.71 with a range from 2 to 7. The polymorphism information content（PIC） for the SSR loci varied from 0.157 to 0.813 with an average of 0.564. Genetic similarities among the 37 inbred lines ranged from 0.69 to 0.89. The cluster analysis showed that the inbred lines could be classified into 6 distinct clusters. The clustering results were consistent with the groups based on the available pedigree. Most of the parents used in commercial maize hybrids came from the opposite clusters.