用Stock6杂交诱导的单倍体鉴定方法初探

Study on Identifying Methods of Maize Haploid Induced by Stock6

试验以Stock6杂交诱导种子为材料,对单倍体鉴定方法进行初步研究。结果表明,表型性状如子粒Navajo标记、紫色植株标记、株高都可用于鉴定单倍体,综合应用这些方法时株高鉴定最准确。子粒Navajo标记和紫色植株标记鉴定可淘汰掉大量杂交二倍体;在无紫色植株标记的绿色植株中,株高相对较矮的是单倍体,生长发育正常的植株可能是纯合二倍体或杂交二倍体,需进一步通过单倍体子代果穗子粒Navajo标记鉴定,所获子粒均无子粒Navajo标记的是纯合二倍体,有子粒Navajo标记的是杂交二倍体;细胞学鉴定直接验证了其它表型鉴别方法的鉴定准确性。总之,这些单倍体鉴定方法各有优缺点,综合应用它们可以提高单倍体鉴定效率,加快单倍体育种技术在玉米遗传育种中的应用。

The identifying methods of maize haploid were studied preliminarily with the materials induced by Stock6. The results showed that all the phenotype methods, such as kernel Navajo marker, purple plant marker andplant height, can be used for identifying haploid. Plant height is the method of extreme nicety to choose haploid. Both kernel Navajo marker and purple plant marker might eliminate some hybrid diploids through selection or contest. In the entire non-purple plant marker＇, haploid is short obviously while those hybrid diploids or homozygous diploids are growing up normally. And the kernel Navajo marker in haploid offspring is the other assistant method to identify. And the hybrid diploids seed have kernel Navajo marker but the diploid have riot. To take count of chromosome is the most reliable source to identify haploid or diploid or polyploid not inch ides homozygous diploid. In total, all the methods have their fineness or defect, and we must use all the methods to identify the haploid that inducing by Stock6 up to now, especially plant height that is the only way for forepart choosing, haploid can be used in maize breeding immediately.