摩擦禾与玉蜀黍属内物种间的分子显带分析

Molecular Banding Analysis Among Tripsacum Dactyloides and Several Species in Senus Zea

分子显带是一种研究物种间共享的重复序列在染色体上分布特征的简便方法,它是通过比较基因组原位杂交进行的。本研究中,我们以摩擦禾基因组DNA为探针,对玉蜀黍属的几个种的染色体进行了比较基因组原位杂交分析。在玉蜀黍属的几个种中都检测到了信号明显的4种带,分别为中间带(interstitialband)、着丝粒带(cen-tromericband)、端粒带(telomericband)和染色纽(knob)。通过分子显带与DAPI显带比较,阐明了供试种间保守的重复DNA序列的分布特征。

Molecular banding undertaken with comparative genomic in s itu hybridization（cGISH） is a simple approach which generates chromosome characteristic signals in heterologous FISH experiments at regions with conserved repeated sequences. In this study, cGISH was undertaken with labeled total genomic DNA of Tripsacum dactyloides （2n=72） to chromosomes of seweral species in genus Zea. Four kinds of bands including interstitial band, centromeric band, telomeric band and knob were detected on all species. Compared to DAPI banding, the distributional characteristics of conserved repeated DNA sequences were illustrated among tested species.