利用醇溶蛋白、盐溶蛋白和RAPD标记划分玉米自交系类群的比较研究

Heterotic Grouping Comparison of Maize Inbred Lines Based on Zeins,Salt-soluble Proteins and RAPD Markers

利用醇溶蛋白、盐溶蛋白和RAPD分子标记研究了16个玉米自交系的遗传多样性,并对这16个自交系进行聚类分析。结果显示,在供试材料中筛选到多态性、重复性均比较好的随机引物13个,检测到醇溶蛋白、盐溶蛋白和RAPD标记的多态性条带分别为30条、34条和112条,分别占总带数的83.3%、85.0%和86.8%。3种标记所得遗传相似系数的相关性均达到了极显著水平,所得平均遗传相似系数(0.597、0.541、0.554)基本一致,RAPD标记的遗传相似系数标准差最小(0.080),相对较可靠。依据醇溶蛋白、盐溶蛋白和RAPD分子标记分别将16个供试自交系划分为3类、3类和4类,聚类分析结果都与系谱分析大体相似,同时也存在与所知系谱不符的现象。

Zeins, salt-soluble proteins and RAPD markers were used to detect the genetic diversity among 16 maize inbred lines, based on which the heterotic groups were classified. 13 random primers were identified with polymorphism and reproducibility among the entries. The numbers of polymorphic bands of zeins, salt-soluble proteins and RAPD makers were 30, 34, and 112 correspondingly, which were about 83.3%, 85.0% and 86.8% of total alleles respectively. A comparison of genetic similarity matrices revealed that the estimates of correlation coefficients based on zeins, salt-soluble proteins and RAPD markers were significantly correlated. The average genetic similarity coefficients of the three markers（0.597, 0.541, 0.554 ） were consistent with each other, but the standard deviation of the one based on RAPD markers was the smallest. These inbred lines were classified into three, three and four groups respectively based on zeins, salt-soluble proteins and RAPD markers data, which were all consistent with the grouping based on the available pedigree data, except for some discordance.