摘要
ＴＮＰ－４７０是半合成的烟曲霉素类似物，具有较强的抗血管生成活性，为了验证其抗癌作用，本文将Ｗａｌｋｅｒ２５６肉瘤细胞接种于Ｗｉｓａｔｒ大鼠皮下，将５０只大鼠随机分成Ａ、Ｂ、Ｃ、Ｄ、Ｅ５组。第二天起分别给予生理盐水。ＴＮＰ－４７０、ＣＴＸＳ．Ｃ．，ｑ．ｏ．ｄ．第九天时，Ａ、Ｂ、Ｃ、Ｄ、Ｅ各组皮下瘤重分别为３．８８±１．２４、２．２０±０．２５、０．８６±０．３０，１．１７±０．３３克。
TNP-470,a semisynthetic analogue of fumagillin has a potent antiangiogenesis effects. In order to test its anti-tumor effect, Walker 256 carcinoma cells(2×107/rat)were inoculated to 50 Wistar rats which were randomized into 5 groups. From the 2nd day.S. C. administration of NS (control), THP-470 (30, 50, 100mg/kg),CTX(30mg/kg)were given to each group.On the 9th day,the mean weight of the subcutanuous tumors of each group was 3. 88±1. 24,2. 20±0. 58,1.64±0. 25,0. 86±0. 30,1.17±0. 33(g) respectively,an anti-tumor effect of TNP-470 was clearly demonstrated in a dose dependent manner when analysized by Stu-dent-Newman-keuls test. Pathological study of the tumors showed that compared with the other 2 groups the number of tumor blood vessels was significantly decreased in TNP-470 treated groups;The extent of tumor necrosis was more severe in the former than the latter. These results suggest that the anti-tumor effect of TNP-470 was mediated by its anti-angiogenesis function.
