西咪替丁抑制人肠癌裸鼠移植瘤

Cimetidine inhibits the growth and angiogenesis of human colon cancer xenograft in nude mice

目的：研究西咪替丁对人结肠癌裸鼠移植瘤生长的抑制作用及机制。方法：建立人结肠癌裸鼠皮下移植瘤模型。随机分2组,每组5只实验鼠。肿瘤种植前3 d开始分别皮下注射生理盐水(对照组)或西咪替丁(治疗组),每天1次,观察成瘤时间及瘤体成长情况。肿瘤种植后第7周处死实验鼠,测定瘤体大小,并用免疫组化方法测定肿瘤组织内微血管密度(MVD)和血管内皮生长因子(VEGF)的表达。结果：治疗组肿瘤体积明显小于对照组；治疗组肿瘤组织中的VEGF表达程度和MVD计数亦明显低于对照组。结论：西咪替丁通过抑制VEGF表达,减少血管生成,从而抑制肿瘤生长。

Objective To investigate the inhibitory effects of cimetidine on the growth of human colon carcinoma xenograft in the nude mouse, and its mechanism. Methods Human colon cancer cell line LoVo cells were subcutaneously inoculated into the BALB/C mice. Ten nude mice were randomly allocated into 2 groups （5 mice in each group）. Either saline or cimetidine [20 mg/（kg·day）] was subcutaneously administrated daily starting three days prior to tumor cell inoculation. The growth of xenografts were monitored. The mice were sacrificed 7 weeks after cell implantation, and immunohistochemistry staining was performed to determine microvessel density （MVD） and expression of vascular endothelial growth factor （VEGF） in tumor tissues. Results The size of the tumors in the exprimental group was significantly smaller than those in the control group, and so was the expression of VEGF and MVD value. Conclusions Cimetidine may inhibit the growth and angiogenesis of colon cancer xenograft by inhibiting VEGF expression.