期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

肺癌细胞系内皮蛋白C受体的表达及其意义 被引量：3

Expression of Endothelial Protein C Receptor in Human Lung Cancer Cell Lines and It's Significance
下载PDF
导出
摘要 目的从细胞、蛋白和基因三个水平分析内皮蛋白C受体(endothelial protein C receptor,EPCR)在肺癌细胞系的表达。方法以EA.hy926内皮细胞作阳性对照,人胚肺WI38细胞作阴性对照,通过流式细胞术、West-ern blot及半定量逆转录-聚合酶链反应分析EPCR在6种肺癌细胞系的表达水平。结果6株肺癌细胞系均有EPCR的表达,高转移肺癌95D细胞表达最高。结论EPCR的表达是肺癌细胞的重要特征,可能与肺癌增殖及转移有关。 Objective To investigate the expression of endothelial protein C receptor（EPCR） in variety of human lung cancer cell lines. Method With EA. hy926 cell as the positive controls and hu- man embryonic lung WI38 as the negative controls, the expression of EPCR in 6 lung cancer cell lines were measured by FCM, Western blot and semi - quantitative reverse transcriptase polymerase chain reaction. Results EPCR was found in all 6 lung cancer cell lines and its most expression was seen in the high - metastasis lung carcinoma 95D cell line. Conclusion Highexpression of EPCR in tumor cell lines provides a potential biological marker for malignancies. It is likely to participate in cell proliferation and metastasis.
作者 衡伟 王兆钺 白霞 戴兰 沈文红 董宁征
机构地区 苏州大学附属一院江苏省血液病研究所
出处 《苏州大学学报（医学版）》 CAS 北大核心 2006年第5期788-792,共5页 Suzhou University Journal of Medical Science
关键词 肺癌细胞 内皮蛋白C受体 流式细胞术 WESTERN BLOT 逆转录-聚合酶链反应 lung cancer cells endothelial protein C receptor FCM Western blot reverse transcriptase polymerase chain reaction
分类号 R734.2 [医药卫生—肿瘤]
  • 相关文献

参考文献10

  • 1Joyce DE,Gelbert L,Ciaccia A,et al.Gene expression profile of antithrombotic protein C defines new mechanisms modulating inflammation and apoptosis[J].J Biol Chem,2001,276:11199-111203.
  • 2Li W,Zheng X,Gu J,et al.Overexpressing endothelial cell protein C receptor alters the hemostatic balance and protects mice from endotoxin[J].J Thromb Haemost,2005,3:1351-1359.
  • 3Tsuneyoshi,N,Fukudome.K,Horiguchi S,et al.Expression and anticoagulant function of the endothelial cell protein C receptor(EPCR)in cancer cell lines[J].Thromb Haemost,2001,85:356-361.
  • 4Scheffer GL,Flens MJ,Hageman S,et al.Expression of the vascular endothelial cell protein C receptor in epithelial tumor cells[J].Eur J Cancer,2002,38:1535-1542.
  • 5Hataji O,Taguchi O,Gabazza EC,et al.Activation of protein C pathway in the airways[J].Lung,2002,180:47-59.
  • 6Cheng T,Liu D,Griffin JH,et al.Activated protein C block p53-mediated apoptosis in ischemmic human brain endithelium and is neuroprotective[J].Nat Med,2003,9:338-342.
  • 7Riewald M,Petrvan RJ,Donner A,et al.Activation of endothelial cell protease activated receptor 1 by the protein C pathway[J].Science,2002,296:1880-1882.
  • 8Uchiba M,Okajima K,Oike Y,et al.Activated protein C induces endothelial cell proliferation by mitogen-activated protein kinase activation in vitro and angiogenesis in vivo[J].Circ Res,2004,95:34-4121.
  • 9Nguyen M,Arkell J,Jackson CJ.Activated protein C directly activates human endothelial gelatinase A[J].J Biol Chem,2000,275:9095-9098.
  • 10Xue M,Thompson P,Kelso I,et al.Activated protein C stimulates proliferation,migration and wound closure,inhibits apoptosis and upregulates MMP-2 activity in cultured human keratinocytes[J].Exp Cell Res,2004,299:119-127.

同被引文献38

  • 1衡伟,王兆钺.内皮蛋白C受体及其病理与临床意义[J].国际输血及血液学杂志,2006,29(1):9-12. 被引量：3
  • 2FUKUDOME K, ESMON CT. Molecular cloning and expression of murine and bovin endothelial cell protein C/activated protein C receptor[J]. J Bio Chem, 1995, 270(10): 5571-5577.
  • 3BEAULIEU LM, CHURCH FC. Activated protein C promotes breast cancer cell migration through interactions with EPCR and PAR-I[J]. Exp Cell Res, 2007, 313(4): 677-687.
  • 4GRAMLING MW, BEAULIEU LM, CHURCH FC. Activated pro- tein C enhances cell motility of endothelial cells and MDA-MB- 231 breast cancer cells by intracellular signal transduction[J]. Exp Cell Res, 2010, 316(3): 314-328.
  • 5BEZUHLY M, CULLEN R, ESMON CT, et al. Role of activated protein C and its receptor in inhibition of tumor metastasis[J]. Blood, 2009, 113(14): 3371-3374.
  • 6GRAMLING MW, BEAULIEU LM, CHURCH FC. Activated pro- tein C enhances cell motility of endothelial cells and MDA-MB- 231 breast cancer cells by intracellular signal transduction [d]. Exp Cell Res, 2010, 316(3): 314-328.
  • 7KESHAVA S, SAHOO S, TUCKER TA, et al. Endothelial cell protein C receptor opposes mesothelioma growth driven by tissue factor[J]. Cancer Res, 2013, 73: 3963.
  • 8GRAMLING MW, BEAULIEU LM, CHURCH FC. Activated protein C enhances cell motility of endothelial cells and MDA- MB-231 breast cancer cells by intracellular signal trmlsduc6on [J]. Exp Cell Res, 2010, 316(3): 314-328.
  • 9Fukudome K,Esmon CT. Molecular eloning and expres- sion of routine and bovin endothelial cell protein C/acti- vated protein C receptor[J]. J Bio Chem, 1995,270( 10): 5571-5577.
  • 10Tsuneyoshi N, Fukudome K, Horiguchi S,et al. Expression and anticoagulant function of the endothelial cell protein C receptor(EPCR)in cancer cell lines[J]. Thmmb Haemosf, 2001,85(2) : 356-361.

引证文献3

二级引证文献2

苏州大学学报（医学版）
2006年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部