胰腺癌组织生长抑素受体mRNA的表达及意义

Expression of Somatostatin Receptor mRNA in Human Pancreatic Cancer and Its Signification

目的 研究人体胰腺癌组织生长抑素受体mRNA的表达。方法 应用逆转录PCR（RT-PCR）检测30例胰腺癌组织和12例正常胰腺组织生长抑素受体亚型mRNA的表达。结果 （1）胰腺癌生长抑素受体5种亚型SSTR1～5mRNA表达依次为2，29±0.87，3，72±1．42，1，91±0.92，1．80±0．98，2.25±0.92，其中SSTR2mRNA表达明显高于其他亚型（P〈0.01）。（2）正常胰腺组织SSTR1～5mRNA表达依次为1.37±0．74，1.38±0．98，1.26±0．79，1．21±0．42，1．33±0.92，各亚型mRNA表达比较无显著性差异（P〉0.05）。（3）胰腺癌SsTR1·2·5mRNA表达明显高于正常胰腺组织的相应亚型（P〈0.01），SSTR3mRNA表达也明显高于正常胰腺组织（P〈0.05）。结论 胰腺癌组织SSTR1·2·3·5mRNA表达水平明显高于正常胰腺组织，并且胰腺癌以SSTR2mRNA表达最为显著。

Objective To investigate the expression of somatostatin receptor（SSTR）mRNA in human pancreatic cancer. Methods The expression of SSTR subtype 1 - 5 mRNA was determined by reverse transcription polymerase chain reaction method in 30 cases of pancreatic cancer and in 12 cases of normal pancreas tissues. Results （1） The expression of SSTR 1- 5 mRNA in pancreatic cancer was 2.29 ± 0.87, 3.72±1.42, 1.91 ±0.92, 1.80 ± 0.98 and 2.25± 0.92. The expression of SSTR2 mRNA was significantly higher than the expression of SSTR1·3· 4·5mRNA（ P 〈 0.01）. （2） The expression of SSTR 1- 5 mRNA in normal pancreas tissues was 1.37 ±0.74, 1.38±0.98, 1.26 ±0.79, 1.21± 0.42 and 1.33± 0.92 respectively. There was no significant difference between the expression of SSTR 1-5 mRNA（ P 〉 0.05）. （3） The expression of SSTR1·2· 5 mRNA in pancreatic cancer was significantly higher than those in the normal pancreas tissues（ P 〈 0.01）. The expression of SSTR3 mRNA was showed also in this way （ P 〈 0.05）. Conclusions The expression of SSTR1·2· 3· 5mRNA in pancreatic cancer is significantly higher than those in the＇normal pancreas tissues. The expression of SSTR2 mRNA is significantly higher than that of SSTR1·3· 4· 5mRNA in pancreatic cancer.