薄层扫描法测定人参降糖胶囊中AF的含量

Quantitative Determination of AF in Ginseng Anti-Diabetes Capsule by TLC Scanning

采用薄层扫描法测定人参降糖胶囊中的精氨酸衍生物AF的含量。以正丁醇-冰醋酸-水（2：1：1）为展开剂，以2g／L茚三酮乙醇溶液为显色剂，AF的测定波长λS=510nm，参比波长λR=685nm。测定结果表明：AF在1．00～3．50μg范围内线性关系良好，r=0．9981。平均回收率99．77％（n=6），RSD=1．34％。

The sample was extracted by methanol at 4℃ and then the residue was extracted by water at 4℃ for 12 h, 3 times altogether, the water extract was freeze-dried and precipitated by ethanol, and the precipitation was dissolved in 5 mL measuring flask. The sample solution was dripped on the silica gel HF 254 plate and outspread by n-butanol,acetic acid, water（2：1 ： 1 ）,2 g/L ninhydrin ethanol solutions was color reagent, AF was determined by thin layer scanning with λS = 510 nm and λR = 685 nm. The results showed that AF has a good linear relation（r =0.998 1） in 1.00- 3.50 μg with the average recovery of 99.77%（ n = 6） and the relative standard deviation of 1.34%.