兔角膜碱烧伤后角膜、房水和晶状体中NO与M DA含量的实验探讨

Levels of nitric oxide and malondialdehyde in the cornea,aqueous humors and len following corneal alkali burn in rabbit: Experimental study

目的:探讨碱烧伤后角膜、房水、晶状体组织中一氧化氮(NO)与丙二醛(M DA)含量改变及其意义。方法:选择健康新西兰大白兔20只(40眼),经麻醉后,用直径9mm圆形滤纸浸透2mm o l/L氢氧化钠,贴附于兔右眼角膜中央区,制作碱烧伤模型。72h后处死,取角膜、房水、晶状体,角膜及晶状体做组织匀浆,用比色法测量其NO和M DA的含量,左眼为正常对照组。结果:碱烧伤72h后角膜、房水、晶状体中NO和M DA含量较对照组显著升高(P<0.01),M DA及NO各组中的含量经过方差分析均不相同,对角膜、房水、晶状体中M DA及NO进行相关性分析,两者为正相关关系。结论:NO、M DA均参与了碱烧伤后眼部损伤的病理生理过程。

Objective： To explore the levels of nitric oxide （NO） and malondialdehyde （MDA） in the cornea, aqueous humors and lens following corneal alkali burn and their significance. Methods.. Twenty healthy rabbits without eye disease were selected, and the corneal alkali burn model of the rabbit riggt eye was prepared by using 2 mol/L NaOH solution to burn the cornea for 60 seconds and then rinsing it for 2 minutes with 0. 9% Sodium Chloride. 72 hours after that the rabbits were killed and the right comea, aqueous humors and lens were taken, with the left eyes as control group. The NO and MDA levels were measured with colorimetry. Results： The NO and MDA levels in the cornea, aqueous humors and lens following corneal alkali burn were significantly higher as compared with those in the control group （P〈0.01）. The NO and MDA levels in all groups of corneal alkali burn were analyzed by using analysis of variance, showing significant difference. The NO level was positively correlated with MDA level in the cornea, aqueous humors and lens following corneal alkali burn. Conclusion： NO and MDA play an important role in the pathophysiological process following corneal alkali burn of rabbit model.