多种细胞因子对人骨髓基质细胞生长的影响

The effects of several cytokines on the growth of human bone marrow stromal cells

目的:探讨多种细胞因子对骨髓基质细胞生长的影响。方法:分离人骨髓单个核细胞,经培养获得骨髓基质细胞(BMSC),分别用不同的细胞因子组合作用于BMSC,MTT法测细胞增殖。采用Mini MACs系统阳性分离纯化脐血CD34+细胞,用BMSC结合不同的细胞因子组合培养14天后计数CFU-Mix集落数。结果r:hGM-CSF结合FL、SCFI﹑L-6对BMSC生长的增殖作用最明显;BMSC联合SCFI、L-3、FL、G-CSF、EPO对促进CD34+细胞体外集落形成的作用最明显。结论:细胞因子作用下的BMSC扩增有可能应用于造血损伤修复。

objective：To detect the effects of cytokines on the growth of human bone marrow stromal cells. Methods：Mononuelear cells were isolated from human bone marrow and cultivated to acquire bone marrow stromal cells, different cytokines in combination were added and prolifieation of BMSC was detected by MTT method, CD34^＋ cells were isolated from umbilical cord blood with Mini MACs system and cultivated with BMSC in combination with different eytokines ,and on the 14th day, the number of CFU-Mix was counted. Results：The most significant promoting effect on the growth of BMSC was observed when rhGM-CSF was added with FL,SCF ,and IL-6.BMSC plus SCF,IL-3 ,FL,G-CSF and EPO was the most effective combination which increased colony formation of CD34^＋ cells. Conclnsions：The results indicate that some eytokines have promoting effects on the expansion of stromal cells in vitro which may have important significance on the repairing of hematopoietie injury.