谷氨酸对大鼠小脑薄片顶核神经元放电的影响

Effect of glutamate on neuronal discharge of fastigial nucleus in rat cerebellar slices

目的:探讨谷氨酸在小脑顶核神经元中的调节作用。方法:应用大鼠小脑薄片,细胞外记录神经元单位放电,观察谷氨酸对顶核神经元单位放电的影响。结果:谷氨酸对小脑顶核神经元放电有兴奋作用,且这种兴奋作用不被低Ca2+/高Mg2+的人工脑脊液所阻断。结论:谷氨酸对小脑顶核神经元的兴奋作用可能是通过激活顶核神经元谷氨酸受体实现的。

Objective：Effect of-glutamate on the discharge of neurons in cerebellar fastigial nucleus was investigated in rat cerebellar slices. Methods ：The cerebellar slices were prepared and spontaneous discharges of single cerebellar fastigial nuclear neurons were recorded by using extracellular recording technique. Results： Glutamate evoked a significant increase in firing rate of the cerebellar fastigial nuclear neurons. A low-Ca^2＋/high-Mg^2＋ medium did not block the excitatory response of cerebellar interpositus nuclear neurons to glutamate. Conclusion：The excitatory effect of glutamate on the discharge of interpositus nuclear neurons in cerebellar slices may be directly mediated by glutamate receptors on interpositus nuclear neurons.