黄石地区慢性无症状乙型肝炎病毒携带者的基因型分布

The distribution of genotypes in chronic asymptomatic hepatitis B virus carriers in Huangshi

目的：了解湖北黄石地区慢性无症状乙型肝炎病毒（HBV）携带者HBV基因型的分布，及其与病毒复制水平、HBeAg表达的相关性。方法：选择黄石地区168例慢性无症状HBV携带者作为研究对象，HBV基因型采用PCR微板核酸杂交．ELISA方法检测；血清HBVDNA复制水平采用荧光定量PCR检测；HBV—M采用ELISA法检测。结果：168例慢性无症状HBV携带者中HBVDNA阳性者为114例（阳性率为67．9％），其基因型分布为B、C、D型以及这3种基因型组成的混合型，而未发现A、E、F基因型。其中以B型、C型为主，所占比例为62．6％和36．8％，D型及混合型比例均为5．3％。C基因型患者中，HBV DNA呈现高水平复制（10^7～10^8 Copies／ml）的患者比例为26．2％（11／42），B基因型为13．3％（8／60）（P〈0．05）。C基因型患者血清抗-HBe阳性率（40．5％）显著高于B基因型（15．0％）（P〈0．01）。结论：黄石地区存在HBV的B、C、D基因型，以及由它们组成的混合基因型；B基因型为优势基因型；C基因型与HBV高复制水平，以及基因变异相关。

Obiective： This study investigated the distribution of HBV genotypes and their correlation with levels of HBV replication and genovariation in chronic asymptomatic HBV carriers in Huangshi, Hubei province. Methods： One hundred and sixty-eight chronic asymptomatic HBV carriers were selected as research objects. The HBV genotypes were detected by PCR microplate hybridization ELISA. The levels of serum HBV DNA replication were determined by fluorescent quantitative PCR. The serum HBV markers were assayed by ELISA. Results： The patients whose HBV DNA were positive were 114 among these 168 chronic HBV carriers （ rate was 67. 9% ） . The genotypes B and C were predominant in 114 patients, and the ration were 62. 6% and 36. 8% respectively. The genotypes A, E and F were not found in these patients. In the genotype C patients, whoes levels of HBV replication presented high （ 10^7-10^8 copies/ml）, accounted for 26. 2%, while genotype B patients accounted for 13.3% （P 〈 0. 05） . The serum anti-HBe positive rate （40. 5% ） in genotype C patients was obviously higher than that of genotype B patients （P 〈 0. 01 ） . Conclusions： The results suggested the HBV genotype B, C, D and their mixed genotype were existed in Huangshi area. Genotype B was the dominant type. Genotype C was correlated with the high HBV replication levels and genovariation.