青霉素对体外培养鼠脑γ－氨基丁酸能神经元及胶质细胞的影响

The effect of convulsant PEN on cultured GABA-energic neurons and GFAP-like immunoreactivity astrocytes of rat cortex and hippocampus

以免疫细胞化学方法观察了分离培养４天、１０天鼠大脑皮层、海马ＧＡＢＡ能神经元以及ＧＦＡＰ免疫反应阳性星形胶质细胞对大剂量致痫剂青霉素（Ｐｅｎｉｃｉｌｉｎ，ＰＥＮ）的反应。结果大剂量ＰＥＮ（３００μ／ｍｌ培养液）可引起培养海马及皮层γ－氨基丁酸（γ－ａｍｉｎｏｂｕｔｙｒｉｃａｃｉｄ，ＧＡＢＡ）能神经元数目明显减少，星形胶质细胞显著增生，且尤以海马胶质细胞增生明显。提示：（１）脑内尤其海马区星形胶质细胞增生与癫痫发生、发展有一定的关系；（２）传统致痫剂ＰＥＮ可能是通过抑制ＧＡＢＡ能神经元功能、刺激星形胶质细胞增生。

To determine the effect of high dose of convulsant penicillin (PEN) on GABA-energic neurons and astrocytes ,the neurons and astrocytes of fetal rat cortex and hippocampus cultured for 4 day and 10 day were studied by means of immunocytochemistry .The results showed that the number of GABA-energic neurons of cortex and hippocampus apparently decreased .The GFAP-like immunoreactivity astrocytes ,especically in hippocampus Dramatically proliferated in the 300μ/ml PEN group comparing with control group.The role of GABA and astrocytes in pathogenesis of epilepsy were discussed in the present paper.